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raav2 retro cag cre  (Addgene inc)


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    Addgene inc raav2 retro cag cre
    a ., h ., r ., w . Experimental design. b-f . Representative epifluorescent image of a coronal slice of brain injected with an AAV2-DIO-eif1a-eYFP anterograde virus in the Insula showing the injection site in the insula (b, c) and projections to the contralateral insula (d), the dlBNST (e), the CeA (f). Scale 100 µm for b,d,e,f and 50 µm for c. g. Quantification of the Insula interhemispheric projections in bilateral dlBNST, CeA, and contralateral Insula. i , j . Representative epifluorescent image of a coronal slice of brain injected with a <t>rAAV2-retro-CAG-Cre</t> retrograde virus in the ipsilateral insula in AI9dTomato mouse (i, scale 500 µm) with a high magnification of contralateral labeling in Insula (j, scale 25 µm). k , l . Quantification of contralateral labeling in Insula (homotopic labeling) and other cortical regions (heterotopic labeling). m-p . Immunofluorescence confocal images showing Insula interhemispheric neurons (tomato labeling, m), insula Satb2 staining (yellow labeling, n), insula Ctip2 labeling (green labeling, o), and the overlay at low (top, scale 100 µm) and high magnification (bottom, scale 25 µm). At the bottom, white arrows show examples of Tomato and Satb2 colocalizations. q . Quantification of Tomato, Satb2, and Ctip2 colocalization in the Insula. s . Representative traces showing a collision test and a high-frequency stimulation protocol for an Insula interhemispheric neuron projecting to the other Insula . t . Histogram of the onset latency of Insula antidromic responses. u , v . Representative epifluorescent image at low (left, scale bar 500 µm) and high magnification (right, 150 µm) of MBP staining (grey labeling) and Insula interhemispheric neurons (tomato labeling). x , y . Representative image obtained with electron microscopy showing immunogold GFP labeling of unmyelinated interhemispheric Insula axons passing through the corpus callosum (x) or the anterior commissure (y) (green arrow). White arrow shows an example of a myelinated axon (scale 500 nm). dlBNST: dorsolateral bed nucleus of the stria terminalis; ovBNST: oval-BNST; juxta-BNST: juxtacapsular BNST; a . c .: anterior commissure; cc . corpus callosum; BLA: basolateral amygdala; CeA: central amygdala; Ins: Insula; Som: Somatosensory cortex; M1: primary Motor cortex; M2:secondary Motor cortex; mPFC: medial Prefrontal cortex; Rec: recording; MBP: myelin basic protein . n: number of neurons; N: number of mice.
    Raav2 Retro Cag Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/raav2+retro+cag+cre/rAAV2-retro+(Plasmid+%2383283)/bio_rxiv__2022__12__15__520538-142-0-9
    Average 94 stars, based on 34 article reviews
    raav2 retro cag cre - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Encoding social preference by interhemispheric neurons in the Insula"

    Article Title: Encoding social preference by interhemispheric neurons in the Insula

    Journal: bioRxiv

    doi: 10.1101/2022.12.15.520538

    a ., h ., r ., w . Experimental design. b-f . Representative epifluorescent image of a coronal slice of brain injected with an AAV2-DIO-eif1a-eYFP anterograde virus in the Insula showing the injection site in the insula (b, c) and projections to the contralateral insula (d), the dlBNST (e), the CeA (f). Scale 100 µm for b,d,e,f and 50 µm for c. g. Quantification of the Insula interhemispheric projections in bilateral dlBNST, CeA, and contralateral Insula. i , j . Representative epifluorescent image of a coronal slice of brain injected with a rAAV2-retro-CAG-Cre retrograde virus in the ipsilateral insula in AI9dTomato mouse (i, scale 500 µm) with a high magnification of contralateral labeling in Insula (j, scale 25 µm). k , l . Quantification of contralateral labeling in Insula (homotopic labeling) and other cortical regions (heterotopic labeling). m-p . Immunofluorescence confocal images showing Insula interhemispheric neurons (tomato labeling, m), insula Satb2 staining (yellow labeling, n), insula Ctip2 labeling (green labeling, o), and the overlay at low (top, scale 100 µm) and high magnification (bottom, scale 25 µm). At the bottom, white arrows show examples of Tomato and Satb2 colocalizations. q . Quantification of Tomato, Satb2, and Ctip2 colocalization in the Insula. s . Representative traces showing a collision test and a high-frequency stimulation protocol for an Insula interhemispheric neuron projecting to the other Insula . t . Histogram of the onset latency of Insula antidromic responses. u , v . Representative epifluorescent image at low (left, scale bar 500 µm) and high magnification (right, 150 µm) of MBP staining (grey labeling) and Insula interhemispheric neurons (tomato labeling). x , y . Representative image obtained with electron microscopy showing immunogold GFP labeling of unmyelinated interhemispheric Insula axons passing through the corpus callosum (x) or the anterior commissure (y) (green arrow). White arrow shows an example of a myelinated axon (scale 500 nm). dlBNST: dorsolateral bed nucleus of the stria terminalis; ovBNST: oval-BNST; juxta-BNST: juxtacapsular BNST; a . c .: anterior commissure; cc . corpus callosum; BLA: basolateral amygdala; CeA: central amygdala; Ins: Insula; Som: Somatosensory cortex; M1: primary Motor cortex; M2:secondary Motor cortex; mPFC: medial Prefrontal cortex; Rec: recording; MBP: myelin basic protein . n: number of neurons; N: number of mice.
    Figure Legend Snippet: a ., h ., r ., w . Experimental design. b-f . Representative epifluorescent image of a coronal slice of brain injected with an AAV2-DIO-eif1a-eYFP anterograde virus in the Insula showing the injection site in the insula (b, c) and projections to the contralateral insula (d), the dlBNST (e), the CeA (f). Scale 100 µm for b,d,e,f and 50 µm for c. g. Quantification of the Insula interhemispheric projections in bilateral dlBNST, CeA, and contralateral Insula. i , j . Representative epifluorescent image of a coronal slice of brain injected with a rAAV2-retro-CAG-Cre retrograde virus in the ipsilateral insula in AI9dTomato mouse (i, scale 500 µm) with a high magnification of contralateral labeling in Insula (j, scale 25 µm). k , l . Quantification of contralateral labeling in Insula (homotopic labeling) and other cortical regions (heterotopic labeling). m-p . Immunofluorescence confocal images showing Insula interhemispheric neurons (tomato labeling, m), insula Satb2 staining (yellow labeling, n), insula Ctip2 labeling (green labeling, o), and the overlay at low (top, scale 100 µm) and high magnification (bottom, scale 25 µm). At the bottom, white arrows show examples of Tomato and Satb2 colocalizations. q . Quantification of Tomato, Satb2, and Ctip2 colocalization in the Insula. s . Representative traces showing a collision test and a high-frequency stimulation protocol for an Insula interhemispheric neuron projecting to the other Insula . t . Histogram of the onset latency of Insula antidromic responses. u , v . Representative epifluorescent image at low (left, scale bar 500 µm) and high magnification (right, 150 µm) of MBP staining (grey labeling) and Insula interhemispheric neurons (tomato labeling). x , y . Representative image obtained with electron microscopy showing immunogold GFP labeling of unmyelinated interhemispheric Insula axons passing through the corpus callosum (x) or the anterior commissure (y) (green arrow). White arrow shows an example of a myelinated axon (scale 500 nm). dlBNST: dorsolateral bed nucleus of the stria terminalis; ovBNST: oval-BNST; juxta-BNST: juxtacapsular BNST; a . c .: anterior commissure; cc . corpus callosum; BLA: basolateral amygdala; CeA: central amygdala; Ins: Insula; Som: Somatosensory cortex; M1: primary Motor cortex; M2:secondary Motor cortex; mPFC: medial Prefrontal cortex; Rec: recording; MBP: myelin basic protein . n: number of neurons; N: number of mice.

    Techniques Used: Injection, Labeling, Immunofluorescence, Staining, Electron Microscopy

    Related Articles

    Plasmid Preparation:

    Article Title: Encoding social preference by interhemispheric neurons in the Insula
    Article Snippet: rAAV2-retro-CAG-Cre (2,8x10 vg/mL; UNC Vector Core, Boyden);AAV2.2-eif1a-DIO-eYFP (3x10 vg/mL; Addgene); AAV2.5-eif1a-DIO-eYFP (1x10 vg/mL; Addgene);AAV2.2-hSyn-eYFP (3x10 vg/mL; 50465-AAV2,Addgene); AAV2.5—eif1a-DIO-eYFP (1x10 vg/mL; 27056-AAV5, Addgene); AAV2.2-hSyn-ChR2(H134R)-eYFP (3.1x10 vg/mL; UNC, AV4384G); AAV5-flex-taCasp3-TEVp (7x10 vg/mL; Addgene); Tetrodotoxin (TTX, 0.5 μM, abcam ab120055); 4 aminopyridine (4AP; 1 mM, ascent scientific, asc-122-100mg)

    Injection:

    Article Title: Encoding social preference by interhemispheric neurons in the Insula
    Article Snippet: rAAV2-retro-CAG-Cre (2,8x10 vg/mL; UNC Vector Core, Boyden);AAV2.2-eif1a-DIO-eYFP (3x10 vg/mL; Addgene); AAV2.5-eif1a-DIO-eYFP (1x10 vg/mL; Addgene);AAV2.2-hSyn-eYFP (3x10 vg/mL; 50465-AAV2,Addgene); AAV2.5—eif1a-DIO-eYFP (1x10 vg/mL; 27056-AAV5, Addgene); AAV2.2-hSyn-ChR2(H134R)-eYFP (3.1x10 vg/mL; UNC, AV4384G); AAV5-flex-taCasp3-TEVp (7x10 vg/mL; Addgene); Tetrodotoxin (TTX, 0.5 μM, abcam ab120055); 4 aminopyridine (4AP; 1 mM, ascent scientific, asc-122-100mg)

    Labeling:

    Article Title: Encoding social preference by interhemispheric neurons in the Insula
    Article Snippet: rAAV2-retro-CAG-Cre (2,8x10 vg/mL; UNC Vector Core, Boyden);AAV2.2-eif1a-DIO-eYFP (3x10 vg/mL; Addgene); AAV2.5-eif1a-DIO-eYFP (1x10 vg/mL; Addgene);AAV2.2-hSyn-eYFP (3x10 vg/mL; 50465-AAV2,Addgene); AAV2.5—eif1a-DIO-eYFP (1x10 vg/mL; 27056-AAV5, Addgene); AAV2.2-hSyn-ChR2(H134R)-eYFP (3.1x10 vg/mL; UNC, AV4384G); AAV5-flex-taCasp3-TEVp (7x10 vg/mL; Addgene); Tetrodotoxin (TTX, 0.5 μM, abcam ab120055); 4 aminopyridine (4AP; 1 mM, ascent scientific, asc-122-100mg)

    Immunofluorescence:

    Article Title: Encoding social preference by interhemispheric neurons in the Insula
    Article Snippet: rAAV2-retro-CAG-Cre (2,8x10 vg/mL; UNC Vector Core, Boyden);AAV2.2-eif1a-DIO-eYFP (3x10 vg/mL; Addgene); AAV2.5-eif1a-DIO-eYFP (1x10 vg/mL; Addgene);AAV2.2-hSyn-eYFP (3x10 vg/mL; 50465-AAV2,Addgene); AAV2.5—eif1a-DIO-eYFP (1x10 vg/mL; 27056-AAV5, Addgene); AAV2.2-hSyn-ChR2(H134R)-eYFP (3.1x10 vg/mL; UNC, AV4384G); AAV5-flex-taCasp3-TEVp (7x10 vg/mL; Addgene); Tetrodotoxin (TTX, 0.5 μM, abcam ab120055); 4 aminopyridine (4AP; 1 mM, ascent scientific, asc-122-100mg)

    Staining:

    Article Title: Encoding social preference by interhemispheric neurons in the Insula
    Article Snippet: rAAV2-retro-CAG-Cre (2,8x10 vg/mL; UNC Vector Core, Boyden);AAV2.2-eif1a-DIO-eYFP (3x10 vg/mL; Addgene); AAV2.5-eif1a-DIO-eYFP (1x10 vg/mL; Addgene);AAV2.2-hSyn-eYFP (3x10 vg/mL; 50465-AAV2,Addgene); AAV2.5—eif1a-DIO-eYFP (1x10 vg/mL; 27056-AAV5, Addgene); AAV2.2-hSyn-ChR2(H134R)-eYFP (3.1x10 vg/mL; UNC, AV4384G); AAV5-flex-taCasp3-TEVp (7x10 vg/mL; Addgene); Tetrodotoxin (TTX, 0.5 μM, abcam ab120055); 4 aminopyridine (4AP; 1 mM, ascent scientific, asc-122-100mg)

    Electron Microscopy:

    Article Title: Encoding social preference by interhemispheric neurons in the Insula
    Article Snippet: rAAV2-retro-CAG-Cre (2,8x10 vg/mL; UNC Vector Core, Boyden);AAV2.2-eif1a-DIO-eYFP (3x10 vg/mL; Addgene); AAV2.5-eif1a-DIO-eYFP (1x10 vg/mL; Addgene);AAV2.2-hSyn-eYFP (3x10 vg/mL; 50465-AAV2,Addgene); AAV2.5—eif1a-DIO-eYFP (1x10 vg/mL; 27056-AAV5, Addgene); AAV2.2-hSyn-ChR2(H134R)-eYFP (3.1x10 vg/mL; UNC, AV4384G); AAV5-flex-taCasp3-TEVp (7x10 vg/mL; Addgene); Tetrodotoxin (TTX, 0.5 μM, abcam ab120055); 4 aminopyridine (4AP; 1 mM, ascent scientific, asc-122-100mg)



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    raav2 retro cag cre  (Addgene inc)
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    Addgene inc raav2 retro cag cre
    a ., h ., r ., w . Experimental design. b-f . Representative epifluorescent image of a coronal slice of brain injected with an AAV2-DIO-eif1a-eYFP anterograde virus in the Insula showing the injection site in the insula (b, c) and projections to the contralateral insula (d), the dlBNST (e), the CeA (f). Scale 100 µm for b,d,e,f and 50 µm for c. g. Quantification of the Insula interhemispheric projections in bilateral dlBNST, CeA, and contralateral Insula. i , j . Representative epifluorescent image of a coronal slice of brain injected with a <t>rAAV2-retro-CAG-Cre</t> retrograde virus in the ipsilateral insula in AI9dTomato mouse (i, scale 500 µm) with a high magnification of contralateral labeling in Insula (j, scale 25 µm). k , l . Quantification of contralateral labeling in Insula (homotopic labeling) and other cortical regions (heterotopic labeling). m-p . Immunofluorescence confocal images showing Insula interhemispheric neurons (tomato labeling, m), insula Satb2 staining (yellow labeling, n), insula Ctip2 labeling (green labeling, o), and the overlay at low (top, scale 100 µm) and high magnification (bottom, scale 25 µm). At the bottom, white arrows show examples of Tomato and Satb2 colocalizations. q . Quantification of Tomato, Satb2, and Ctip2 colocalization in the Insula. s . Representative traces showing a collision test and a high-frequency stimulation protocol for an Insula interhemispheric neuron projecting to the other Insula . t . Histogram of the onset latency of Insula antidromic responses. u , v . Representative epifluorescent image at low (left, scale bar 500 µm) and high magnification (right, 150 µm) of MBP staining (grey labeling) and Insula interhemispheric neurons (tomato labeling). x , y . Representative image obtained with electron microscopy showing immunogold GFP labeling of unmyelinated interhemispheric Insula axons passing through the corpus callosum (x) or the anterior commissure (y) (green arrow). White arrow shows an example of a myelinated axon (scale 500 nm). dlBNST: dorsolateral bed nucleus of the stria terminalis; ovBNST: oval-BNST; juxta-BNST: juxtacapsular BNST; a . c .: anterior commissure; cc . corpus callosum; BLA: basolateral amygdala; CeA: central amygdala; Ins: Insula; Som: Somatosensory cortex; M1: primary Motor cortex; M2:secondary Motor cortex; mPFC: medial Prefrontal cortex; Rec: recording; MBP: myelin basic protein . n: number of neurons; N: number of mice.
    Raav2 Retro Cag Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/raav2+retro+cag+cre/rAAV2-retro+(Plasmid+%2383283)/bio_rxiv__2022__12__15__520538-142-0-9
    Average 94 stars, based on 1 article reviews
    raav2 retro cag cre - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    a ., h ., r ., w . Experimental design. b-f . Representative epifluorescent image of a coronal slice of brain injected with an AAV2-DIO-eif1a-eYFP anterograde virus in the Insula showing the injection site in the insula (b, c) and projections to the contralateral insula (d), the dlBNST (e), the CeA (f). Scale 100 µm for b,d,e,f and 50 µm for c. g. Quantification of the Insula interhemispheric projections in bilateral dlBNST, CeA, and contralateral Insula. i , j . Representative epifluorescent image of a coronal slice of brain injected with a rAAV2-retro-CAG-Cre retrograde virus in the ipsilateral insula in AI9dTomato mouse (i, scale 500 µm) with a high magnification of contralateral labeling in Insula (j, scale 25 µm). k , l . Quantification of contralateral labeling in Insula (homotopic labeling) and other cortical regions (heterotopic labeling). m-p . Immunofluorescence confocal images showing Insula interhemispheric neurons (tomato labeling, m), insula Satb2 staining (yellow labeling, n), insula Ctip2 labeling (green labeling, o), and the overlay at low (top, scale 100 µm) and high magnification (bottom, scale 25 µm). At the bottom, white arrows show examples of Tomato and Satb2 colocalizations. q . Quantification of Tomato, Satb2, and Ctip2 colocalization in the Insula. s . Representative traces showing a collision test and a high-frequency stimulation protocol for an Insula interhemispheric neuron projecting to the other Insula . t . Histogram of the onset latency of Insula antidromic responses. u , v . Representative epifluorescent image at low (left, scale bar 500 µm) and high magnification (right, 150 µm) of MBP staining (grey labeling) and Insula interhemispheric neurons (tomato labeling). x , y . Representative image obtained with electron microscopy showing immunogold GFP labeling of unmyelinated interhemispheric Insula axons passing through the corpus callosum (x) or the anterior commissure (y) (green arrow). White arrow shows an example of a myelinated axon (scale 500 nm). dlBNST: dorsolateral bed nucleus of the stria terminalis; ovBNST: oval-BNST; juxta-BNST: juxtacapsular BNST; a . c .: anterior commissure; cc . corpus callosum; BLA: basolateral amygdala; CeA: central amygdala; Ins: Insula; Som: Somatosensory cortex; M1: primary Motor cortex; M2:secondary Motor cortex; mPFC: medial Prefrontal cortex; Rec: recording; MBP: myelin basic protein . n: number of neurons; N: number of mice.

    Journal: bioRxiv

    Article Title: Encoding social preference by interhemispheric neurons in the Insula

    doi: 10.1101/2022.12.15.520538

    Figure Lengend Snippet: a ., h ., r ., w . Experimental design. b-f . Representative epifluorescent image of a coronal slice of brain injected with an AAV2-DIO-eif1a-eYFP anterograde virus in the Insula showing the injection site in the insula (b, c) and projections to the contralateral insula (d), the dlBNST (e), the CeA (f). Scale 100 µm for b,d,e,f and 50 µm for c. g. Quantification of the Insula interhemispheric projections in bilateral dlBNST, CeA, and contralateral Insula. i , j . Representative epifluorescent image of a coronal slice of brain injected with a rAAV2-retro-CAG-Cre retrograde virus in the ipsilateral insula in AI9dTomato mouse (i, scale 500 µm) with a high magnification of contralateral labeling in Insula (j, scale 25 µm). k , l . Quantification of contralateral labeling in Insula (homotopic labeling) and other cortical regions (heterotopic labeling). m-p . Immunofluorescence confocal images showing Insula interhemispheric neurons (tomato labeling, m), insula Satb2 staining (yellow labeling, n), insula Ctip2 labeling (green labeling, o), and the overlay at low (top, scale 100 µm) and high magnification (bottom, scale 25 µm). At the bottom, white arrows show examples of Tomato and Satb2 colocalizations. q . Quantification of Tomato, Satb2, and Ctip2 colocalization in the Insula. s . Representative traces showing a collision test and a high-frequency stimulation protocol for an Insula interhemispheric neuron projecting to the other Insula . t . Histogram of the onset latency of Insula antidromic responses. u , v . Representative epifluorescent image at low (left, scale bar 500 µm) and high magnification (right, 150 µm) of MBP staining (grey labeling) and Insula interhemispheric neurons (tomato labeling). x , y . Representative image obtained with electron microscopy showing immunogold GFP labeling of unmyelinated interhemispheric Insula axons passing through the corpus callosum (x) or the anterior commissure (y) (green arrow). White arrow shows an example of a myelinated axon (scale 500 nm). dlBNST: dorsolateral bed nucleus of the stria terminalis; ovBNST: oval-BNST; juxta-BNST: juxtacapsular BNST; a . c .: anterior commissure; cc . corpus callosum; BLA: basolateral amygdala; CeA: central amygdala; Ins: Insula; Som: Somatosensory cortex; M1: primary Motor cortex; M2:secondary Motor cortex; mPFC: medial Prefrontal cortex; Rec: recording; MBP: myelin basic protein . n: number of neurons; N: number of mice.

    Article Snippet: rAAV2-retro-CAG-Cre (2,8x10 vg/mL; UNC Vector Core, Boyden);AAV2.2-eif1a-DIO-eYFP (3x10 vg/mL; Addgene); AAV2.5-eif1a-DIO-eYFP (1x10 vg/mL; Addgene);AAV2.2-hSyn-eYFP (3x10 vg/mL; 50465-AAV2,Addgene); AAV2.5—eif1a-DIO-eYFP (1x10 vg/mL; 27056-AAV5, Addgene); AAV2.2-hSyn-ChR2(H134R)-eYFP (3.1x10 vg/mL; UNC, AV4384G); AAV5-flex-taCasp3-TEVp (7x10 vg/mL; Addgene); Tetrodotoxin (TTX, 0.5 µM, abcam ab120055); 4 aminopyridine (4AP; 1 mM, ascent scientific, asc-122-100mg)

    Techniques: Injection, Labeling, Immunofluorescence, Staining, Electron Microscopy